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KMID : 1059519990430010069
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Journal of the Korean Chemical Society
1999 Volume.43 No. 1 p.69 ~ p.73
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Allosteric Activator Site Study by Time Dependent Kinetic Behavior of Aspartase from Hafnia alvei
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Paik Tae-Jin
Lee Min-Sub
Han Tae-Su
Yoon Moon-Young
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Abstract
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A time lag was observed during the amination reaction of Hafnia alvei aspartase. The time lag was shortened at lower pH, but lengthened at higher pH. Also, the time lag was shortened at higher temperature, lengthened at lower temperature. L-aspartate, D-aspartate and α-methyl-DL-aspartate markedly revealed the activating effects. The time lag and the nonlinear kinetics were eliminated when one of these effectors was added in the reaction mixture. These results suggest that these effectors occupy a distant allosteric activator site apart from active site.
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KEYWORD
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